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| Arruda,E.Z.; Silva,N.M.V.; Moraes,R.A.M.; Melo,P.A.. |
| We investigated the protective effect of suramin, an enzyme inhibitor and an uncoupler of G protein from receptors, on the myotoxic activity in mice of different crotalid snake venoms (A.c. laticinctus, C.v. viridis, C.d. terrificus, B. jararacussu, B. moojeni, B. alternatus, B. jararaca, L. muta). Myotoxicity was evaluated in vivo by injecting im the venoms (0.5 or 1.0 mg/kg) dissolved in physiological saline solution (0.1 ml) and measuring plasma creatine kinase (CK) activity. Two experimental approaches were used in mice (N = 5 for each group). In protocol A, 1 mg of each venom was incubated with 1.0 mg suramin (15 min, 37ºC, in vitro), and then injected im into the mice at a dose of 1.0 mg/kg (in vivo). In protocol B, venoms, 1.0 mg/kg, were injected... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Snake venom; Myotoxicity; Creatine kinase; Suramin. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000600013 |
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| Bueno,L. G. F.; Leite,G. B.; Cruz-Höfling,M. A.; Rodrigues-Simioni,L.; Oshima-Franco,Y.. |
| In the present study, manganese (Mn2+), a neuromuscular blocker with pre and postsynaptic actions, was used to verify the neurotoxicity and myotoxicity induced by Crotalus durissus terrificus (Cdt) and Bothrops jararacussu (Bjssu) venoms in biventer cervicis preparations (BCp). Preparations pretreated with 0.66 and 1.6mM Mn2+ did not affect Cdt venom-induced blockage nor change KCl-induced contracture but partially reduced ACh-induced contracture. However, both Mn2+ concentrations partially hindered Bjssu venom-induced blockage after washing the preparations with Krebs solution, and only 1.6mM Mn2+ preparations significantly recovered ACh-induced contracture. The effect of Cdt venom myotoxicity on contractile responses was different from that of Bjssu... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Contracture; Divalent cation; Mn2+; Myotoxicity; Neuromuscular blockage; Neurotoxicity. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000200007 |
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| BORJA-OLIVEIRA,C. R.; SOARES,A. M.; ZAMUNÉR,S. R.; HYSLOP,S.; GIGLIO,J. R.; PRADO-FRANCESCHI,J.; RODRIGUES-SIMIONI,L.. |
| Snake venoms frequently vary in composition. In this work, we compared the neurotoxic and myotoxic activities of 16 lots of Bothrops neuwiedii venoms from different regions of Brazil, using chick biventer cervicis preparations. The neuromuscular blockade varied from 2% to 100% after 120 min incubation with venoms (50 mug/ml). In all cases, this blockade was irreversible and concentration-dependent; at low concentrations (10-20 mug/ml), 15 of the 16 venom lots failed to abolish responses to acetylcholine (110 muM), but blocked responses to KCl (13.4 muM), and induced contracture. At 5-20 mug/ml, the most active venom totally blocked twitch-tension without affecting responses to acetylcholine and KCl. Polyacrylamide gel electrophoresis for basic proteins... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bothrops neuwiedii venom; Intraspecific variation; Myotoxicity; Neurotoxicity; Neuromuscular blockade. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302002000100008 |
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| Durigon,A. M.; Borja-Oliveira,C. R.; Dal Belo,C. A.; Oshima-Franco,Y.; Cogo,J. C.; Lapa,A. J.; Souccar,C.; Rodrigues-Simioni,L.. |
| The pharmacological effects of Bothrops neuwiedi pauloensis venom on mouse phrenic nerve-diaphragm (PND) preparations were studied. Venom (20 mug/ml) irreversibly inhibited indirectly evoked twitches in PND preparations (60 ± 10% inhibition, mean ± SEM; p<0.05; n=6). At 50 mug/ml, the venom blocked indirectly and directly (curarized preparations) evoked twitches in mouse hemidiaphragms. In the absence of Ca2+, venom (50 mug/ml), produced partial blockade only after an 80 min incubation, which reached 40.3 ± 7.8% (p<0.05; n=3) after 120 min. Venom (20 mug/ml) increased (25 ± 2%, p< 0.05) the frequency of giant miniature end-plate potentials in 9 of 10 end-plates after 30 min and the number of miniature end-plate potentials which was maximum (562 ±... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Giant MEPPs; Myotoxicity; Neurotoxicity; Presynaptic action. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000100004 |
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| Charoenpitakchai,Mongkon; Wiwatwarayos,Kulachet; Jaisupa,Nattapon; Rusmili,Muhamad Rusdi Ahmad; Mangmool,Supachoke; Hodgson,Wayne C.; Ruangpratheep,Chetana; Chanhome,Lawan; Chaisakul,Janeyuth. |
| Abstract Background: Envenoming by kraits (genus Bungarus) is a medically significant issue in South Asia and Southeast Asia. Malayan krait (Bungarus candidus) venom is known to contain highly potent neurotoxins. In recent years, there have been reports on the non-neurotoxic activities of krait venom that include myotoxicity and nephrotoxicity. However, research on such non-neurotoxicity activities of Malayan krait venom is extremely limited. Thus, the aim of the present study was to determine the myotoxic, cytotoxic and nephrotoxic activities of B. candidus venoms from northeastern (BC-NE) and southern (BC-S) Thailand in experimentally envenomed rats. Methods: Rats were administered Malayan krait (BC-NE or BC-S) venom (50 μg/kg, i.m.) or 0.9% NaCl... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Krait; Bungarus candidus; Myotoxicity; Nephrotoxicity; Venom; Kidney. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100304 |
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| Saturnino-Oliveira,J.; Tomaz,M.A.; Fonseca,T.F.; Gaban,G.A.; Monteiro-Machado,M.; Strauch,M.A.; Cons,B.L.; Calil-Elias,S.; Martinez,A.M.B.; Melo,P.A.. |
| We studied the effect of pulsed ultrasound therapy (UST) and antibothropic polyvalent antivenom (PAV) on the regeneration of mouse extensor digitorum longus muscle following damage by Bothrops jararacussu venom. Animals (Swiss male and female mice weighing 25.0 ± 5.0 g; 5 animals per group) received a perimuscular injection of venom (1 mg/kg) and treatment with UST was started 1 h later (1 min/day, 3 MHz, 0.3 W/cm², pulsed mode). Three and 28 days after injection, muscles were dissected and processed for light microscopy. The venom caused complete degeneration of muscle fibers. UST alone and combined with PAV (1.0 mL/kg) partially protected these fibers, whereas muscles receiving no treatment showed disorganized fascicules and fibers with reduced diameter.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pulsed therapeutic ultrasound; Bothrops snake venom; Myotoxicity; Mouse skeletal-muscle regeneration. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000600003 |
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| Borja-Oliveira,C.R.; Durigon,A.M.; Vallin,A.C.C.; Toyama,M.H.; Souccar,C.; Marangoni,S.; Rodrigues-Simioni,L.. |
| The neuromuscular effects of Bothrops neuwiedii pauloensis (jararaca-pintada) venom were studied on isolated chick biventer cervicis nerve-muscle preparations. Venom concentrations of 5-50 µg/ml produced an initial inhibition and a secondary increase of indirectly evoked twitches followed by a progressive concentration-dependent and irreversible neuromuscular blockade. At venom concentrations of 1-20 µg/ml, the responses to 13.4 mM KCl were inhibited whereas those to 110 µM acetylcholine alone and cumulative concentrations of 1 µM to 10 mM were unaffected. At venom concentrations higher than 50 µg/ml, there was pronounced muscle contracture with inhibition of the responses to acetylcholine, KCl and direct stimulation. At 20-24ºC, the venom (50 µg/ml)... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chick biventer cervicis; Myotoxicity; Neurotoxicity; Phospholipase A2; Presynaptic action. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000500009 |
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